Journal:

Article Title: Cyclin-dependent kinase 5 phosphorylates signal transducer and activator of transcription 3 and regulates its transcriptional activity

doi: 10.1073/pnas.0307606100

Figure Lengend Snippet: Cdk5 regulated the STAT3 DNA-binding and transcriptional activity in vitro and in vivo. (A) Blockade of Cdk5 activity by Ros inhibited the NRG-induced increase in DNA binding of STAT3 in C2C12 myotubes. (Lower) Total STAT3. (Right) DNA-binding activity of STAT3 α and β was quantitated and expressed as a ratio of that in control myotubes pretreated with DMSO. Each value represented the mean ± SEM. of three representative experiments; *, P < 0.005. (B) Northern blot analysis of c-fos and junB in primary muscle cultures prepared from E18.5 wild-type (+/+) or Cdk5 null (-/-) embryos with (+) or without (-) NRG treatment. (Upper) c-fos. (Lower) junB. (C) DNA binding of STAT3 in E18.5 Cdk5 wild-type (+/+) and mutant (-/-) muscle (Left) and quantitation (Right). DNA-binding activity of STAT3 α and β was quantitated and expressed as a ratio of that in wild-type muscle. Each value represented the mean ± SEM. of three representative experiments; *, P < 0.005. (D) Northern blot analysis of fibronectin and muscle creatine kinase (MCK) in wild-type (+/+) and Cdk5-deficient (-/-) muscle of E18.5 embryos. MCK served as an equal loading control. (E) Overexpression of p35 increased the transcriptional activity of STAT3 in primary chick myotubes in a dose-dependent manner. Primary chick myotubes were transiently transfected with a STAT3 (pSTAT3-TA-Luc) or STAT1 (pGAS-TA-Luc) reporter gene construct and an internal control plasmid (β-gal-pCMV) with or without p35 or dnCdk5. The ratio of DNAs used for transfection was as depicted on the x axis. Luciferase activity was measured and normalized against the β-gal activity in the samples. Promoter activity was expressed as the ratio of luciferase activity in cells transfected with different combinations of p35 and dnCdk5 relative to that transfected with the empty vector. The data represented the mean ± SEM, n = 5. (F) Overexpression of p35 increased the transcriptional activity of c-fos. c-fos promoter-luciferase assay was performed as described in E with pSTAT3-TA-Luc (pSTAT3) as a positive control. Preincubation with Ros reduced Cdk5/p35-mediated transcriptional activity of pSTAT3 or c-fos. Overexpression of p35 could not increase the luciferase reporter activity under the control of STAT1 enhancer-responsive element (pGAS). The data represented mean ± SEM, n = 3. (G) The increase of STAT3 transcriptional activity depends on Ser-727 phosphorylation of STAT3. Primary muscle cultures were transfected with indicated expression constructs and luciferase assay was performed as in E; mean ± SEM, n = 3.

Article Snippet: Transfection mixtures consisted of a mixture of pSTAT3-TA-Luc, pGL3-c-fos, or pGAS reporter together with p35 or the following cDNA constructs (dnCdk5, STAT3, or STAT3M) and β-galactosidase (βgal)pCMV constructs (Clontech).

Techniques: Binding Assay, Activity Assay, In Vitro, In Vivo, Northern Blot, Mutagenesis, Quantitation Assay, Over Expression, Transfection, Construct, Plasmid Preparation, Luciferase, Positive Control, Expressing

Journal: Ophthalmic epidemiology

Article Title: Comparative effectiveness of generic latanoprost versus branded prostaglandin analogs for primary open angle glaucoma

doi: 10.1080/09286586.2018.1516786

Figure Lengend Snippet: Baseline demographic information for “intention-to-treat” analysis in A) generic v. the branded form of latanoprost and B) generic latanoprost v. all branded PGAs in POAG patients initiated on treatment after 2010:

Article Snippet: 5 , 6 Among PGAs, latanoprost is the most frequently prescribed, having both a branded (Xalatan®, Pfizer, New York, NY) and generic versions available.

Techniques: Biomarker Discovery

Journal: Ophthalmic epidemiology

Article Title: Comparative effectiveness of generic latanoprost versus branded prostaglandin analogs for primary open angle glaucoma

doi: 10.1080/09286586.2018.1516786

Figure Lengend Snippet: Baseline demographic information after propensity score matching:

Article Snippet: 5 , 6 Among PGAs, latanoprost is the most frequently prescribed, having both a branded (Xalatan®, Pfizer, New York, NY) and generic versions available.

Techniques: